IMMUNE PROPERTIES IN THE PRODUCTED RECOMBINANT SIP YOUNG 6 HIS PROTEIN FROM THE SEA STAR IGKAPPA GENE THROUGH HEK 293 EBNA CELLS REVEALING AGAINST SPIKE-RBD PROTEIN AND HRP ONE CHANGES IN SEA STAR GENE EXPRESSION?

Main Article Content

Michel Leclerc

Abstract

In 2014 we have isolated and cloned the sea star Igkappa gene which induced an anti-HRP primitive invertebrate antibody. We attempt, in the present work, to produce a SIP Young 6 His protein through HeK 293 EBNA cells after Igkappa gene cloning. This protein bound HRP by the use of Escherichia coli as vector of cloning but not HRP with HeK cells as vector. Is there changes in sea star IGKappa gene expression? We notice also that the SARS-CoV-2-SPIKE RBD Protein didn’t bind to SIP Young 6 His protein when compared to HRP.


 

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Article Details

How to Cite
Leclerc, M. . (2021). IMMUNE PROPERTIES IN THE PRODUCTED RECOMBINANT SIP YOUNG 6 HIS PROTEIN FROM THE SEA STAR IGKAPPA GENE THROUGH HEK 293 EBNA CELLS REVEALING AGAINST SPIKE-RBD PROTEIN AND HRP ONE CHANGES IN SEA STAR GENE EXPRESSION?. American International Journal of Biology and Life Sciences, 3(1), 28–32. https://doi.org/10.46545/aijbls.v3i1.309
Section
Articles
Author Biography

Michel Leclerc, University of Orleans, France

Immunology of Invertebrates

Department of Cell Biology, Developmental Biology, Immunology

 University of Orleans, France

References

Kujirai, T., Arimura, Y., Fujita, R., Horikoshi, N., Machida, S., & Kurumizaka, H. (2018). Methods for preparing nucleosomes containing histone variants. In Histone Variants (pp. 3-20). Humana Press, New York, NY.

Leclerc, M., & Otten, P. (2014). Immune Properties Corroborated by A. Rubens Sea Star Igkappa Gene. SAJ Biotechnology, 1(1), 104-105.

Leclerc, M. (2021). Recombinant Sip Young 6 His Protein Production from the Sea Star Igkappa Gene, after Cloning. European Journal of Biology and Biotechnology, 2(2), 85-86.

Malm, M., Saghaleyni, R., Lundqvist, M., Giudici, M., Chotteau, V., Field, R., & Rockberg, J. (2020). Evolution from adherent to suspension: systems biology of HEK293 cell line development. Scientific reports, 10(1), 1-15.

Osteras, N. V. M., Otten, P., & Leclerc, M. A. (2014). A new gene in Asterias rubens : a sea star IGKappa gene Meta gene 2(3), 20-22.